CRISPR/Cas9 ribonucleoprotein-mediated knockin generation in hTERT-RPE1 cells

CRISPR/Cas9 ribonucleoprotein-mediated knockin generation in hTERT-RPE1 cells

Blog Article

Summary: hTERT-RPE1 cells are genetically stable near diploid cells widely used to model cell division, DNA repair, or ciliogenesis in a non-transformed context.However, poor transfectability and limited homology-directed repair capacity hamper their amenability to Bumper Guards and Accessories gene editing.Here, we describe a protocol for rapid and efficient generation of diverse homozygous knockins.In contrast to other approaches, this strategy bypasses the Homeopathics need for molecular cloning.

Our approach can also be applied to a variety of cell types including cancer and induced pluripotent stem cells (iPSCs).